9E88

De novo backtracked transcription elongation complex of Mycobacterium tuberculosis RNA polymerase on a linear DNA fragment (TEC-Backtracked)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (A5U8D3) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P9WGZ1)	Mycobacterium tuberculosis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1177	129905.7	1	UniProt (A5U052) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P9WGY9)	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1333	148883.0	1	UniProt (P0A675) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P9WGY7)	Mycobacterium tuberculosis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (A0A045H2R3) Pfam (PF01192) UniProt (by SIFTS) (P9WGY5)	Mycobacterium tuberculosis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (M)	Ubiquitin-like protein SMT3,RNA polymerase-binding transcription factor CarD	polymer	285	32104.2	1	UniProt (Q12306) UniProt (P9WJG2) Pfam (PF00240) Pfam (PF02559) Pfam (PF21095) UniProt (by SIFTS) (P9WJG3)	Mycobacterium tuberculosis
6	G (N)	DNA (27-MER)	polymer	127	39318.3	1		synthetic construct
7	H (R)	RNA (5'-R(P*GP*CP*GP*AP*GP*AP*GP*GP*GP*AP*CP*AP*CP*G)-3')	polymer	21	6893.2	1		synthetic construct
8	I (T)	DNA (33-MER)	polymer	127	39053.9	1		synthetic construct
9	J, K (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	L (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1316 (UniProt: P0A675)

PDB	External Database	Details
Leu -8	-	expression tag
Ala -7	-	expression tag
Arg -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag

M: -98 - -1 (UniProt: Q12306)

PDB	External Database	Details
Met -122	-	expression tag
Gly -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
His -111	-	expression tag
Ser -110	-	expression tag
Ser -109	-	expression tag
Gly -108	-	expression tag
His -107	-	expression tag
Ile -106	-	expression tag
Glu -105	-	expression tag
Gly -104	-	expression tag
Arg -103	-	expression tag
His -102	-	expression tag
Met -101	-	expression tag
Ala -100	-	expression tag
Ser -99	-	expression tag
Ser 0	-	linker

M: 1 - 162 (UniProt: P9WJG2)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	483500.1
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	483655.2

*Water molecules are not included.