9E86

De Novo Mycobacterium tuberculosis transcription initiation promoter complex with 5-mer RNA and open Beta' clamp (RNA Polymerase with Sigma-A, CarD, and RbpA)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (A5U8D3) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Mycobacterium tuberculosis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1177	129905.7	1	UniProt (A5U052) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1333	148883.0	1	UniProt (P0A675) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Mycobacterium tuberculosis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (A0A045H2R3) Pfam (PF01192)	Mycobacterium tuberculosis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor SigA	polymer	550	60512.0	1	UniProt (P0A603) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Mycobacterium tuberculosis	Sigma-A
6	G (J)	RNA polymerase-binding protein RbpA	polymer	111	12993.7	1	UniProt (P9WHJ4) Pfam (PF13397)	Mycobacterium tuberculosis
7	H (M)	Ubiquitin-like protein SMT3,RNA polymerase-binding transcription factor CarD	polymer	285	32104.2	1	UniProt (Q12306) UniProt (P9WJG2) Pfam (PF00240) Pfam (PF02559) Pfam (PF21095)	Mycobacterium tuberculosis
8	I (N)	DNA (52-MER)	polymer	127	39318.3	1		synthetic construct
9	J (R)	RNA (5'-R(P*AP*GP*CP*GP*A)-3')	polymer	21	6893.2	1		synthetic construct
10	K (T)	DNA (48-MER)	polymer	127	39053.9	1		synthetic construct
11	L (C)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
12	M, N (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
13	O (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1316 (UniProt: P0A675)

PDB	External Database	Details
Leu -8	-	expression tag
Ala -7	-	expression tag
Arg -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag

F: 1 - 528 (UniProt: P0A603)

PDB	External Database	Details
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

M: -98 - -1 (UniProt: Q12306)

PDB	External Database	Details
Met -122	-	expression tag
Gly -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
His -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
His -111	-	expression tag
Ser -110	-	expression tag
Ser -109	-	expression tag
Gly -108	-	expression tag
His -107	-	expression tag
Ile -106	-	expression tag
Glu -105	-	expression tag
Gly -104	-	expression tag
Arg -103	-	expression tag
His -102	-	expression tag
Met -101	-	expression tag
Ala -100	-	expression tag
Ser -99	-	expression tag
Ser 0	-	linker

M: 1 - 162 (UniProt: P9WJG2)

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	557005.8
Non-Polymers*	Number of molecules	4
	Total formula weight	331.1
All*	Total formula weight	557336.9

*Water molecules are not included.