9E7T

Cryo-EM structure of NOT1:NOT7:PieF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Dot/Icm T4SS effector PieF	polymer	150	16568.5	1	UniProt (A0AAN5PHN6) UniProt (by SIFTS) (Q5ZU36)	Legionella pneumophila
2	B (B)	CCR4-NOT transcription complex subunit 1	polymer	254	29237.8	1	UniProt (A5YKK6) Pfam (PF16415)	Homo sapiens (human)	CCR4-associated factor 1,Negative regulator of transcription subunit 1 homolog,NOT1H,hNOT1
3	C (C)	SDH7p Mitochondrial protein involved in assembly of succinate dehydrogenase,CCR4-NOT transcription complex subunit 7	polymer	411	47134.9	1	UniProt (A0A0L8VUM0) UniProt (Q9UIV1) Pfam (PF04857)	Homo sapiens (human)	BTG1-binding factor 1,CCR4-associated factor 1,CAF-1,Caf1a
4	D (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F (A, C)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 1 - 125 (UniProt: A0AAN5PHN6)

PDB	External Database	Details
Met -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

B: 1093 - 1317 (UniProt: A5YKK6)

PDB	External Database	Details
Met 1064	-	expression tag
Gly 1065	-	expression tag
Ser 1066	-	expression tag
Ser 1067	-	expression tag
His 1068	-	expression tag
His 1069	-	expression tag
His 1070	-	expression tag
His 1071	-	expression tag
His 1072	-	expression tag
His 1073	-	expression tag
Ser 1074	-	expression tag
Ser 1075	-	expression tag
Gly 1076	-	expression tag
Thr 1077	-	expression tag
Gly 1078	-	expression tag
Ser 1079	-	expression tag
Gly 1080	-	expression tag
Leu 1081	-	expression tag
Glu 1082	-	expression tag
Val 1083	-	expression tag
Leu 1084	-	expression tag
Phe 1085	-	expression tag
Gln 1086	-	expression tag
Gly 1087	-	expression tag
Pro 1088	-	expression tag
His 1089	-	expression tag
Met 1090	-	expression tag
Leu 1091	-	expression tag
Glu 1092	-	expression tag

C: -105 - -11 (UniProt: A0A0L8VUM0)

PDB	External Database	Details
Met -125	-	expression tag
Gly -124	-	expression tag
Ser -123	-	expression tag
Ser -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
Ser -115	-	expression tag
Ser -114	-	expression tag
Gly -113	-	expression tag
Leu -112	-	expression tag
Val -111	-	expression tag
Pro -110	-	expression tag
Arg -109	-	expression tag
Gly -108	-	expression tag
Ser -107	-	expression tag
His -106	-	expression tag
Thr -10	-	linker
Gly -9	-	linker
Gly -8	-	linker
Ser -7	-	linker
Glu -6	-	linker
Asn -5	-	linker
Leu -4	-	linker
Tyr -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker

C: 1 - 285 (UniProt: Q9UIV1)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	92941.3
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	92965.6

*Water molecules are not included.