9E2Y
Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA replication complex GINS protein PSF1 | polymer | 208 | 24230.6 | 1 | UniProt (Q12488) Pfam (PF05916) | Saccharomyces cerevisiae W303 | Partner of Sld five 1 |
| 2 | B (B) | DNA replication complex GINS protein PSF2 | polymer | 213 | 25096.8 | 1 | UniProt (P40359) Pfam (PF05916) | Saccharomyces cerevisiae W303 | Partner of Sld five 2 |
| 3 | C (C) | DNA replication complex GINS protein PSF3 | polymer | 217 | 24437.9 | 1 | UniProt (Q12146) Pfam (PF22466) Pfam (PF05916) | Saccharomyces cerevisiae W303 | Partner of Sld five 3 |
| 4 | D (D) | DNA replication complex GINS protein SLD5 | polymer | 294 | 33983.6 | 1 | UniProt (Q03406) Pfam (PF05916) Pfam (PF16922) | Saccharomyces cerevisiae W303 | |
| 5 | E (E) | Cell division control protein 45 | polymer | 650 | 74324.8 | 1 | UniProt (Q08032) Pfam (PF02724) | Saccharomyces cerevisiae W303 | |
| 6 | F (F) | Leading strand DNA template | polymer | 35 | 10993.0 | 1 | synthetic construct | ||
| 7 | G (G) | Lagging strand DNA template | polymer | 7 | 2146.4 | 1 | synthetic construct | ||
| 8 | H (2) | DNA replication licensing factor MCM2 | polymer | 868 | 98911.5 | 1 | UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae W303 | Minichromosome maintenance protein 2 |
| 9 | I (3) | DNA replication licensing factor MCM3 | polymer | 971 | 107653.5 | 1 | UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae W303 | Minichromosome maintenance protein 3 |
| 10 | J (4) | DNA replication licensing factor MCM4 | polymer | 933 | 105138.4 | 1 | UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) | Saccharomyces cerevisiae W303 | Cell division control protein 54 |
| 11 | K (5) | Minichromosome maintenance protein 5 | polymer | 775 | 86505.7 | 1 | UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) | Saccharomyces cerevisiae W303 | Cell division control protein 46 |
| 12 | L (6) | DNA replication licensing factor MCM6 | polymer | 1017 | 113110.2 | 1 | UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) | Saccharomyces cerevisiae W303 | Minichromosome maintenance protein 6 |
| 13 | M (7) | DNA replication licensing factor MCM7 | polymer | 845 | 95049.9 | 1 | UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Saccharomyces cerevisiae W303 | Cell division control protein 47,Minichromosome maintenance protein 7 |
| 14 | N (X) | Topoisomerase 1-associated factor 1 | polymer | 43 | 4676.3 | 1 | UniProt (P53840) | Saccharomyces cerevisiae W303 | |
| 15 | O (2) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 16 | AA, DA, P, R, T... (6, 7, 2, 3, 4...) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 17 | BA, EA, Q, U, Y (6, 7, 2, 4, 5) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) | |||
| 18 | CA, S, V, W, Z (7, 4, 5, 6) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 5 | Chemie (ATP) |
Sequence modifications
C: 1 - 194 (UniProt: Q12146)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Ser -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Ser -4 | - | expression tag |
| His -3 | - | expression tag |
| Met -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 806258.7 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 3436.0 | |
| All* | Total formula weight | 809694.7 |
