9E2X

Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 2

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA replication complex GINS protein PSF1	polymer	208	24230.6	1	UniProt (Q12488) Pfam (PF05916)	Saccharomyces cerevisiae W303	Partner of Sld five 1
2	B (B)	DNA replication complex GINS protein PSF2	polymer	213	25096.8	1	UniProt (P40359) Pfam (PF05916)	Saccharomyces cerevisiae W303	Partner of Sld five 2
3	C (C)	DNA replication complex GINS protein PSF3	polymer	217	24437.9	1	UniProt (Q12146) Pfam (PF22466) Pfam (PF05916)	Saccharomyces cerevisiae W303	Partner of Sld five 3
4	D (D)	DNA replication complex GINS protein SLD5	polymer	294	33983.6	1	UniProt (Q03406) Pfam (PF05916) Pfam (PF16922)	Saccharomyces cerevisiae W303
5	E (E)	Cell division control protein 45	polymer	650	74324.8	1	UniProt (Q08032) Pfam (PF02724)	Saccharomyces cerevisiae W303
6	F (F)	Leading strand DNA template	polymer	48	15060.6	1		synthetic construct
7	G (G)	Lagging strand DNA template	polymer	20	6112.0	1		synthetic construct
8	H (2)	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae W303	Minichromosome maintenance protein 2
9	I (3)	DNA replication licensing factor MCM3	polymer	971	107653.5	1	UniProt (P24279) Pfam (PF22207) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae W303	Minichromosome maintenance protein 3
10	J (4)	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Saccharomyces cerevisiae W303	Cell division control protein 54
11	K (5)	Minichromosome maintenance protein 5	polymer	775	86505.7	1	UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933)	Saccharomyces cerevisiae W303	Cell division control protein 46
12	L (6)	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae W303	Minichromosome maintenance protein 6
13	M (7)	DNA replication licensing factor MCM7	polymer	845	95049.9	1	UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae W303	Cell division control protein 47,Minichromosome maintenance protein 7
14	N (X)	Topoisomerase 1-associated factor 1	polymer	1238	141296.9	1	UniProt (P53840) Pfam (PF04821)	Saccharomyces cerevisiae W303
15	O (Y)	Chromosome segregation in meiosis protein 3	polymer	92	10939.9	1	UniProt (Q04659) Pfam (PF07962)	Saccharomyces cerevisiae W303
16	CA, P, S, Y (7, 2, 3, 5)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)
17	DA, Q, T, V, Z (7, 2, 3, 4, 5)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
18	AA, BA, EA, R, W (5, 6, 7, 2, 4)	ZINC ION	non-polymer		65.4	5	Chemie (ZN)
19	U, X (4)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

C: 1 - 194 (UniProt: Q12146)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	961852.3
Non-Polymers*	Number of molecules	16
	Total formula weight	3331.7
All*	Total formula weight	965184.0

*Water molecules are not included.