9DZU
Cryo-EM structure of the C. neoformans lipid flippase Apt1-Cdc50 bound with butyrolactol A in the E2P state
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Transcription regulator | polymer | 411 | 45938.0 | 1 | UniProt (J9VW44) Pfam (PF03381) | Cryptococcus neoformans var. grubii H99 | |
| 2 | B (A) | Phospholipid-transporting ATPase | polymer | 1570 | 176534.6 | 1 | UniProt (J9VZ19) Pfam (PF16209) Pfam (PF13246) Pfam (PF16212) | Cryptococcus neoformans var. grubii H99 | |
| 3 | C, E (D, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 4 | D (E) | alpha-D-mannopyranose-(1-4)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G34220DL) | |||
| 5 | F (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 6 | G (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
| 7 | H (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | I (A) | (3R,4R,5S)-3,4-dihydroxy-5-[(1R,2R,3S,4S,5R,6R,8E,10E,14E,16Z)-1,2,3,4,5-pentahydroxy-6,20,20-trimethylhenicosa-8,10,14,16-tetraen-1-yl]oxolan-2-one | non-polymer | 526.7 | 1 | Chemie (A1BD6) |
Sequence modifications
B: 1 - 401 (UniProt: J9VW44)
A: 1 - 1561 (UniProt: J9VZ19)
| PDB | External Database | Details |
|---|---|---|
| His 402 | - | expression tag |
| His 403 | - | expression tag |
| His 404 | - | expression tag |
| His 405 | - | expression tag |
| His 406 | - | expression tag |
| His 407 | - | expression tag |
| His 408 | - | expression tag |
| His 409 | - | expression tag |
| His 410 | - | expression tag |
| His 411 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp 1562 | - | expression tag |
| Tyr 1563 | - | expression tag |
| Lys 1564 | - | expression tag |
| Asp 1565 | - | expression tag |
| Asp 1566 | - | expression tag |
| Asp 1567 | - | expression tag |
| Asp 1568 | - | expression tag |
| Lys 1569 | - | expression tag |
| Ile 1570 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 222472.6 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1921.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 838.2 | |
| All* | Total formula weight | 225232.5 |
