9DZ6

Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State 3-1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein MSN5	polymer	1230	143067.2	1	UniProt (P52918) Pfam (PF19273)	Saccharomyces cerevisiae (brewer's yeast)
2	B (B)	GTP-binding nuclear protein GSP1/CNR1	polymer	186	21283.5	1	UniProt (P32835) Pfam (PF00071)	Saccharomyces cerevisiae (brewer's yeast)	Chromosome stability protein 17,GTPase Ran homolog,Genetic suppressor of PRP20-1
3	C (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	D (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

A: 1 - 1224 (UniProt: P52918)

PDB	External Database	Details
Glu 1225	-	expression tag
Asn 1226	-	expression tag
Leu 1227	-	expression tag
Tyr 1228	-	expression tag
Phe 1229	-	expression tag
Gln 1230	-	expression tag

B: 2 - 179 (UniProt: P32835)

PDB	External Database	Details
Met 0	-	expression tag
Ala 1	-	expression tag
Leu 71	Gln 71	engineered mutation
Glu 180	-	expression tag
Asn 181	-	expression tag
Leu 182	-	expression tag
Tyr 183	-	expression tag
Phe 184	-	expression tag
Gln 185	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	164350.7
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	164898.2

*Water molecules are not included.