9DZ2

Cryo-EM structure of Sudan ebolavirus glycoprotein complexed with hNPC1-C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (C, D)	NPC intracellular cholesterol transporter 1	polymer	280	31792.5	2	UniProt (O15118) Pfam (PF22314)	Homo sapiens (human)	Niemann-Pick C1 protein
2	B, D, G (I, A, E)	Envelope glycoprotein	polymer	195	21546.8	3	UniProt (Q7T9D9) Pfam (PF01611)	Sudan ebolavirus	GP1,2,GP
3	C, E, H (J, B, F)	Shed GP	polymer	165	18787.2	3	UniProt (Q7T9D9) Pfam (PF22307)	Sudan ebolavirus	GP1,2-delta

Sequence modifications

C, D: 374 - 620 (UniProt: O15118)

PDB	External Database	Details
Met 351	-	initiating methionine
Asp 352	-	expression tag
Ala 353	-	expression tag
Met 354	-	expression tag
Lys 355	-	expression tag
Arg 356	-	expression tag
Gly 357	-	expression tag
Leu 358	-	expression tag
Cys 359	-	expression tag
Cys 360	-	expression tag
Val 361	-	expression tag
Leu 362	-	expression tag
Leu 363	-	expression tag
Leu 364	-	expression tag
Cys 365	-	expression tag
Gly 366	-	expression tag
Ala 367	-	expression tag
Val 368	-	expression tag
Phe 369	-	expression tag
Val 370	-	expression tag
Ser 371	-	expression tag
Ala 372	-	expression tag
Ser 373	-	expression tag
Gly 621	-	expression tag
Ser 622	-	expression tag
Trp 623	-	expression tag
Ser 624	-	expression tag
His 625	-	expression tag
Pro 626	-	expression tag
Gln 627	-	expression tag
Phe 628	-	expression tag
Glu 629	-	expression tag
Lys 630	-	expression tag

J, B, F: 509 - 637 (UniProt: Q7T9D9)

PDB	External Database	Details
Gly 638	-	expression tag
Ser 639	-	expression tag
Gly 640	-	expression tag
Tyr 641	-	expression tag
Ile 642	-	expression tag
Pro 643	-	expression tag
Glu 644	-	expression tag
Ala 645	-	expression tag
Pro 646	-	expression tag
Arg 647	-	expression tag
Asp 648	-	expression tag
Gly 649	-	expression tag
Gln 650	-	expression tag
Ala 651	-	expression tag
Tyr 652	-	expression tag
Val 653	-	expression tag
Arg 654	-	expression tag
Lys 655	-	expression tag
Asp 656	-	expression tag
Gly 657	-	expression tag
Glu 658	-	expression tag
Trp 659	-	expression tag
Val 660	-	expression tag
Leu 661	-	expression tag
Leu 662	-	expression tag
Ser 663	-	expression tag
Thr 664	-	expression tag
Phe 665	-	expression tag
Leu 666	-	expression tag
Gly 667	-	expression tag
His 668	-	expression tag
His 669	-	expression tag
His 670	-	expression tag
His 671	-	expression tag
His 672	-	expression tag
His 673	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	184586.8
All*	Total formula weight	184586.8

*Water molecules are not included.