9DXM

Cryo-EM structure of yeast Exportin Msn5 bound to RanGTP and Pho4 (not modeled) (State 2-1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein MSN5	polymer	1230	143067.2	1	UniProt (P52918) Pfam (PF19273)	Saccharomyces cerevisiae (brewer's yeast)
2	B (B)	GTP-binding nuclear protein GSP1/CNR1	polymer	186	21283.5	1	UniProt (P32835) Pfam (PF00071)	Saccharomyces cerevisiae (brewer's yeast)	Chromosome stability protein 17,GTPase Ran homolog,Genetic suppressor of PRP20-1
3	C (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
4	D (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 1224 (UniProt: P52918)

PDB	External Database	Details
Glu 1225	-	expression tag
Asn 1226	-	expression tag
Leu 1227	-	expression tag
Tyr 1228	-	expression tag
Phe 1229	-	expression tag
Gln 1230	-	expression tag

B: 2 - 179 (UniProt: P32835)

PDB	External Database	Details
Met 0	-	expression tag
Ala 1	-	expression tag
Leu 71	Gln 71	engineered mutation
Glu 180	-	expression tag
Asn 181	-	expression tag
Leu 182	-	expression tag
Tyr 183	-	expression tag
Phe 184	-	expression tag
Gln 185	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	164350.7
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	164898.2

*Water molecules are not included.