9DSN

D306A Mutant of M.tuberculosis MenD (SEPHCHC Synthase)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, D, B, C)	2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase	polymer	574	60024.9	4	UniProt (P9WK11) Pfam (PF02776)	Mycobacterium tuberculosis H37Rv	SEPHCHC synthase,Menaquinone biosynthesis protein MenD
2	E, H, I, J, L... (A, D, B, C)	FORMIC ACID	non-polymer		46.0	7	Chemie (FMT)
3	F, O (D, C)	THIAMINE DIPHOSPHATE	non-polymer		425.3	2	Chemie (TPP)
4	G, P (D, C)	1,4-dihydroxy-2-naphthoic acid	non-polymer		204.2	2	Chemie (DNA)
5	K, R (D, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	N (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	S, T, U, V (A, D, B, C)	water	water		18.0	461	Chemie (HOH)

Sequence modifications

A, D, B, C: 1 - 554 (UniProt: P9WK11)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 306	Asp 306	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	240099.7
Non-Polymers*	Number of molecules	14
	Total formula weight	1665.2
All*	Total formula weight	241764.9

*Water molecules are not included.