9DRJ

Cryo-EM structure of a SUMO E1-E2-SUMO1 complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SUMO-activating enzyme subunit 1	polymer	346	38499.8	1	UniProt (Q9UBE0) Pfam (PF00899)	Homo sapiens (human)	Ubiquitin-like 1-activating enzyme E1A
2	B (B)	SUMO-activating enzyme subunit 2	polymer	548	61230.8	1	UniProt (Q9UBT2) Pfam (PF00899) Pfam (PF14732)	Homo sapiens (human)	Anthracycline-associated resistance ARX,Ubiquitin-like 1-activating enzyme E1B,Ubiquitin-like modifier-activating enzyme 2
3	C (C)	SUMO-conjugating enzyme UBC9	polymer	178	20186.1	1	UniProt (P63279) Pfam (PF00179)	Homo sapiens (human)	RING-type E3 SUMO transferase UBC9,SUMO-protein ligase,Ubiquitin carrier protein 9,Ubiquitin carrier protein I,Ubiquitin-conjugating enzyme E2 I,Ubiquitin-protein ligase I,p18
4	D (D)	Small ubiquitin-related modifier 1	polymer	117	13320.9	1	UniProt (P63165) Pfam (PF11976)	Homo sapiens (human)	SUMO-1,GAP-modifying protein 1,GMP1,SMT3 homolog 3,Sentrin,Ubiquitin-homology domain protein PIC1,Ubiquitin-like protein SMT3C,Smt3C,Ubiquitin-like protein UBL1
5	E (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	F (D)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

C: 1 - 158 (UniProt: P63279)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 138	Cys 138	engineered mutation

D: 1 - 97 (UniProt: P63165)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133237.7
Non-Polymers*	Number of molecules	2
	Total formula weight	412.6
All*	Total formula weight	133650.3

*Water molecules are not included.