9DRJ
Cryo-EM structure of a SUMO E1-E2-SUMO1 complex.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | SUMO-activating enzyme subunit 1 | polymer | 346 | 38499.8 | 1 | UniProt (Q9UBE0) Pfam (PF00899) | Homo sapiens (human) | Ubiquitin-like 1-activating enzyme E1A |
| 2 | B (B) | SUMO-activating enzyme subunit 2 | polymer | 548 | 61230.8 | 1 | UniProt (Q9UBT2) Pfam (PF00899) Pfam (PF14732) | Homo sapiens (human) | Anthracycline-associated resistance ARX,Ubiquitin-like 1-activating enzyme E1B,Ubiquitin-like modifier-activating enzyme 2 |
| 3 | C (C) | SUMO-conjugating enzyme UBC9 | polymer | 178 | 20186.1 | 1 | UniProt (P63279) Pfam (PF00179) | Homo sapiens (human) | RING-type E3 SUMO transferase UBC9,SUMO-protein ligase,Ubiquitin carrier protein 9,Ubiquitin carrier protein I,Ubiquitin-conjugating enzyme E2 I,Ubiquitin-protein ligase I,p18 |
| 4 | D (D) | Small ubiquitin-related modifier 1 | polymer | 117 | 13320.9 | 1 | UniProt (P63165) Pfam (PF11976) | Homo sapiens (human) | SUMO-1,GAP-modifying protein 1,GMP1,SMT3 homolog 3,Sentrin,Ubiquitin-homology domain protein PIC1,Ubiquitin-like protein SMT3C,Smt3C,Ubiquitin-like protein UBL1 |
| 5 | E (B) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | F (D) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40459109) PubChem (46875232) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (177678969) PubChem (6083) PubChem (46874558) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (12358354) PubChem (57879893) PubChem (57879902) PubChem (224) PubChem (122197642) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) |
Sequence modifications
C: 1 - 158 (UniProt: P63279)
D: 1 - 97 (UniProt: P63165)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ser 138 | Cys 138 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 133237.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 412.6 | |
| All* | Total formula weight | 133650.3 |
