9DQB

Cryo-EM structure of a double-loaded SUMO E1-E2-SUMO1 complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	SUMO-conjugating enzyme UBC9	polymer	178	20255.2	1	UniProt (P63279) Pfam (PF00179)	Homo sapiens (human)	RING-type E3 SUMO transferase UBC9,SUMO-protein ligase,Ubiquitin carrier protein 9,Ubiquitin carrier protein I,Ubiquitin-conjugating enzyme E2 I,Ubiquitin-protein ligase I,p18
2	B (D)	Small ubiquitin-related modifier 1	polymer	117	13320.9	1	UniProt (P63165) Pfam (PF11976)	Homo sapiens (human)	SUMO-1,GAP-modifying protein 1,GMP1,SMT3 homolog 3,Sentrin,Ubiquitin-homology domain protein PIC1,Ubiquitin-like protein SMT3C,Smt3C,Ubiquitin-like protein UBL1
3	C (G)	Small ubiquitin-related modifier 1	polymer	117	13288.8	1	UniProt (P63165) Pfam (PF11976)	Homo sapiens (human)	SUMO-1,GAP-modifying protein 1,GMP1,SMT3 homolog 3,Sentrin,Ubiquitin-homology domain protein PIC1,Ubiquitin-like protein SMT3C,Smt3C,Ubiquitin-like protein UBL1
4	D (A)	SUMO-activating enzyme subunit 1	polymer	366	40671.1	1	UniProt (Q9UBE0) Pfam (PF00899)	Homo sapiens (human)	Ubiquitin-like 1-activating enzyme E1A
5	E (B)	SUMO-activating enzyme subunit 2	polymer	548	61230.8	1	UniProt (Q9UBT2) Pfam (PF00899) Pfam (PF14732)	Homo sapiens (human)	Anthracycline-associated resistance ARX,Ubiquitin-like 1-activating enzyme E1B,Ubiquitin-like modifier-activating enzyme 2
6	F (B)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
7	G (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

C: 1 - 158 (UniProt: P63279)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 22	Phe 22	engineered mutation
Gln 23	Gly 23	engineered mutation
Ser 25	Val 25	engineered mutation
Lys 129	Ala 129	engineered mutation
Ser 138	Cys 138	engineered mutation
Arg 153	Lys 153	engineered mutation

D: 1 - 97 (UniProt: P63165)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

G: 1 - 97 (UniProt: P63165)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 52	Cys 52	engineered mutation

A: 1 - 346 (UniProt: Q9UBE0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	148766.9
Non-Polymers*	Number of molecules	2
	Total formula weight	412.6
All*	Total formula weight	149179.5

*Water molecules are not included.