9DO9

Membrane-bound OlyA/PlyB (Hinge-Lock) prepore focused refinement

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, E (A, B, N, O)	Ostreolysin A6	polymer	143	15759.4	4	UniProt (P83467)	Pleurotus ostreatus (oyster mushroom)
2	C, F (C, P)	Pleurotolysin B	polymer	483	53328.7	2	UniProt (Q5W9E8)	Pleurotus ostreatus (oyster mushroom)
3	G, H, I, L, M... (A, B, N, O)	N-oleoyl-D-erythro-sphingosylphosphorylcholine	non-polymer		730.1	12	Chemie (A1A8R)
4	J, K, O, P, T... (A, B, N, O)	CHOLESTEROL	non-polymer		386.7	8	Chemie (CLR)

Sequence modifications

A, B, N, O: 1 - 137 (UniProt: P83467)

PDB	External Database	Details
Ser 62	Cys 62	conflict
Ser 94	Cys 94	conflict
Glu 138	-	expression tag
Asn 139	-	expression tag
Leu 140	-	expression tag
Tyr 141	-	expression tag
Phe 142	-	expression tag
Gln 143	-	expression tag

C, P: 49 - 523 (UniProt: Q5W9E8)

PDB	External Database	Details
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
Val 296	Gly 296	conflict
Val 297	Gly 297	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	169695.0
Non-Polymers*	Number of molecules	20
	Total formula weight	11854.1
All*	Total formula weight	181549.1

*Water molecules are not included.