9DI0
Cryo-EM structure of Kif18A bound to a microtubule
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Kinesin-like protein KIF18A, Methylated-DNA--protein-cysteine methyltransferase chimera | polymer | 562 | 62009.7 | 1 | UniProt (Q8NI77) UniProt (E5BBQ0) Pfam (PF00225) Pfam (PF02870) Pfam (PF01035) | Homo sapiens (human) | Marrow stromal KIF18A,MS-KIF18A,6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase |
| 2 | B (K) | Tubulin beta chain | polymer | 445 | 49907.8 | 1 | UniProt (P02554) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Beta-tubulin |
| 3 | C (L) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 1 | UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain |
| 4 | D (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 5 | E, I (A, L) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | F (K) | TAXOL | non-polymer | 853.9 | 1 | Chemie (TA1) | |||
| 7 | G (K) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 8 | H (L) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) |
Sequence modifications
A: 2 - 374 (UniProt: Q8NI77)
A: 376 - 552 (UniProt: E5BBQ0)
| PDB | External Database | Details |
|---|---|---|
| Val -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Glu -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Glu 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Met 375 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Arg 404 | Glu 33 | conflict |
| Pro 553 | - | expression tag |
| Gly 554 | - | expression tag |
| Leu 555 | - | expression tag |
| Gly 556 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 162121.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2296.1 | |
| All* | Total formula weight | 164418.0 |
