9DGG

ncPRC1RYBP bound to unmodified nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	130	14093.4	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	126	13979.3	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (187-MER)	polymer	187	57982.9	1		synthetic construct
6	J (J)	DNA (187-MER)	polymer	187	57488.6	1		synthetic construct
7	K (K)	Polycomb complex protein BMI-1	polymer	326	37006.1	1	UniProt (P35226) Pfam (PF13923) Pfam (PF16207)	Homo sapiens (human)	Polycomb group RING finger protein 4,RING finger protein 51
8	L (L)	E3 ubiquitin-protein ligase RING2	polymer	336	37706.4	1	UniProt (Q99496) Pfam (PF13923) Pfam (PF16207)	Homo sapiens (human)	Huntingtin-interacting protein 2-interacting protein 3,HIP2-interacting protein 3,Protein DinG,RING finger protein 1B,RING1b,RING finger protein 2,RING finger protein BAP-1,RING-type E3 ubiquitin transferase RING2
9	M, N, O, P (K, L)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, E: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

C, G: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation

D, H: -3 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	299988.5
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	300250.1

*Water molecules are not included.