9D98
Cryo-EM structure of HIV-1 BG505 SOSIP.664 Env bound to 3-sCD4, 3-VRC34.01 Fab with two gp120 protomers rotated, Population 5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Surface protein gp120 | polymer | 469 | 52508.4 | 3 | UniProt (Q2N0S5) Pfam (PF00516) UniProt (by SIFTS) (Q2N0S6) | Human immunodeficiency virus 1 | |
| 2 | D, E, F (D, E, F) | Transmembrane protein gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S5) Pfam (PF00517) UniProt (by SIFTS) (D0PUM0) | Human immunodeficiency virus 1 | |
| 3 | G, I, K (G, I, K) | VRC34.01 Fab heavy chain | polymer | 223 | 23797.6 | 3 | Homo sapiens | ||
| 4 | H, J, L (H, J, L) | VRC34.01 Fab light chain | polymer | 212 | 23138.8 | 3 | Homo sapiens | ||
| 5 | M, N, O (M, N, O) | T-cell surface glycoprotein CD4 | polymer | 181 | 20129.9 | 3 | UniProt (P01730) Pfam (PF00047) Pfam (PF05790) | Homo sapiens (human) | T-cell surface antigen T4/Leu-3 |
| 6 | P (P) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G55220VL) | |||
| 7 | Q (W) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 8 | R (d) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) |
Sequence modifications
A, B, C: 33 - 503 (UniProt: Q2N0S5)
D, E, F: 512 - 664 (UniProt: Q2N0S5)
| PDB | External Database | Details |
|---|---|---|
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 410163.5 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 2732.5 | |
| All* | Total formula weight | 412896.0 |
