9D8Y

Cryo-EM structure of HIV-1 BG505 SOSIP.664 Env bound to 3-sCD4, 3-VRC34.01 Fab with one gp120 rotated, Population 4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Envelope glycoprotein gp160	polymer	469	52508.4	3	UniProt (Q2N0S5) Pfam (PF00516) UniProt (by SIFTS) (Q2N0S6)	Human immunodeficiency virus 1	Env polyprotein
2	D, E, F (D, E, F)	Envelope glycoprotein gp160	polymer	153	17146.5	3	UniProt (Q2N0S6) Pfam (PF00517) UniProt (by SIFTS) (D0PUM0)	Human immunodeficiency virus 1
3	G, I, K (G, I, K)	VRC34.01 Fab Heavy Chain	polymer	223	23797.6	3		Homo sapiens
4	H, J, L (H, J, L)	VRC34.01 Fab Light Chain	polymer	212	23138.8	3		Homo sapiens
5	M, N, O (M, N, O)	T-cell surface glycoprotein CD4	polymer	96	10952.5	3	UniProt (P01730) Pfam (PF00047)	Homo sapiens (human)	T-cell surface antigen T4/Leu-3
6	P, Q, R (P, U, Z)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
7	S (e)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
8	T, U (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A, B, C: 33 - 503 (UniProt: Q2N0S5)

PDB	External Database	Details
Asn 332	Thr 330	engineered mutation
Cys 501	Ala 498	engineered mutation

D, E, F: 512 - 664 (UniProt: Q2N0S6)

PDB	External Database	Details
Pro 559	Ile 556	engineered mutation
Cys 605	Thr 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	382631.2
Branched	Number of molecules	4
	Total formula weight	3156.9
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	386230.5

*Water molecules are not included.