9D85

Structure of the KEOPS complex (Cgi121/Bud32/Kae1/Pcc1) bound to tRNA in a distorted tRNA conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein	polymer	203	23957.5	1	UniProt (Q58530) Pfam (PF01163)	Methanocaldococcus jannaschii
2	B (C)	Regulatory protein Cgi121	polymer	148	16963.9	1	UniProt (Q57646) Pfam (PF08617)	Pyrococcus furiosus DSM 3638	Positive regulator of Bud32 kinase activity
3	C (K)	Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein	polymer	327	36115.7	1	UniProt (Q58530) Pfam (PF00814)	Methanocaldococcus jannaschii
4	D (P)	KEOPS complex subunit Pcc1	polymer	82	9410.9	1	UniProt (Q8TZI1) Pfam (PF09341)	Pyrococcus furiosus DSM 3638
5	E (T)	tRNA	polymer	77	24758.7	1	GenBank (6626255)	Methanocaldococcus jannaschii

Sequence modifications

B: 333 - 535 (UniProt: Q58530)

PDB	External Database	Details
Arg 478	Glu 478	engineered mutation

C: 6 - 150 (UniProt: Q57646)

PDB	External Database	Details
Met 3	-	expression tag
Asp 4	-	expression tag
Pro 5	-	expression tag

K: 1 - 324 (UniProt: Q58530)

PDB	External Database	Details
Met -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

T: 0 - 76 (GenBank: 6626255)

PDB	External Database	Details
C 75	U 863891	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	111206.8
All*	Total formula weight	111206.8

*Water molecules are not included.