9D6F
Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 440-2 and 454-3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (L) | 440-2 Fab light chain | polymer | 221 | 24367.0 | 1 | Mus musculus | ||
| 2 | B (H) | 440-2 Fab heavy chain | polymer | 230 | 24603.3 | 1 | Mus musculus | ||
| 3 | C (A) | 445-3 Fab heavy chain | polymer | 225 | 24124.8 | 1 | Mus musculus | ||
| 4 | D (C) | 445-3 Fab light chain | polymer | 216 | 23697.0 | 1 | Mus musculus | ||
| 5 | E (D) | Type 1 fimbrin D-mannose specific adhesin FimH, Donor strand complemented with FimG peptide 'triple mutant' | polymer | 310 | 32118.6 | 1 | UniProt (P08191) UniProt (P08190) Pfam (PF09160) Pfam (PF00419) | Escherichia coli |
Sequence modifications
D: 1 - 279 (UniProt: P08191)
D: 287 - 300 (UniProt: P08190)
| PDB | External Database | Details |
|---|---|---|
| Ser 7 | Asn 28 | engineered mutation |
| Ala 15 | Gly 36 | engineered mutation |
| Ala 16 | Gly 37 | engineered mutation |
| Ala 27 | Val 48 | engineered mutation |
| Ser 70 | Asn 91 | engineered mutation |
| Gln 228 | Asn 249 | engineered mutation |
| Gly 280 | - | linker |
| Gly 281 | - | linker |
| Ser 282 | - | linker |
| Ser 283 | - | linker |
| Gly 284 | - | linker |
| Gly 285 | - | linker |
| Gly 286 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 301 | - | expression tag |
| Gly 302 | - | expression tag |
| His 303 | - | expression tag |
| His 304 | - | expression tag |
| His 305 | - | expression tag |
| His 306 | - | expression tag |
| His 307 | - | expression tag |
| His 308 | - | expression tag |
| His 309 | - | expression tag |
| His 310 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 128910.8 | |
| All* | Total formula weight | 128910.8 |
