9D45
Cryo-EM structure of yeast Exportin Msn5 bound to cargo Pho4 and RanGTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein MSN5 | polymer | 1230 | 143067.2 | 1 | UniProt (P52918) Pfam (PF19273) | Saccharomyces cerevisiae (brewer's yeast) | |
| 2 | B (B) | GTP-binding nuclear protein GSP1/CNR1 | polymer | 186 | 21283.5 | 1 | UniProt (P32835) Pfam (PF00071) | Saccharomyces cerevisiae (brewer's yeast) | Chromosome stability protein 17,GTPase Ran homolog,Genetic suppressor of PRP20-1 |
| 3 | C (C) | Phosphate system positive regulatory protein PHO4 | polymer | 202 | 22497.4 | 1 | UniProt (P07270) | Saccharomyces cerevisiae (brewer's yeast) | |
| 4 | D (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 5 | E (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 1224 (UniProt: P52918)
B: 2 - 179 (UniProt: P32835)
C: 1 - 200 (UniProt: P07270)
| PDB | External Database | Details |
|---|---|---|
| Glu 1225 | - | expression tag |
| Asn 1226 | - | expression tag |
| Leu 1227 | - | expression tag |
| Tyr 1228 | - | expression tag |
| Phe 1229 | - | expression tag |
| Gln 1230 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
| Glu 180 | - | expression tag |
| Asn 181 | - | expression tag |
| Leu 182 | - | expression tag |
| Tyr 183 | - | expression tag |
| Phe 184 | - | expression tag |
| Gln 185 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 186848.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 187395.6 |
