9D2I

Cryo-EM structure of the Strand displacement Complex (III) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase gamma	polymer	1240	141745.4	1	UniProt (P15801) Pfam (PF18136) Pfam (PF00476)	Saccharomyces cerevisiae (brewer's yeast)	Mitochondrial DNA polymerase catalytic subunit
2	B (P)	Primer DNA	polymer	23	7172.6	1		synthetic construct
3	C (T)	Template DNA	polymer	42	12854.2	1		synthetic construct
4	D (N)	Non-Template DNA	polymer	23	7078.6	1		synthetic construct
5	E (A)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
6	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	G (P)	3'-DEOXYTHYMIDINE-5'-MONOPHOSPHATE	non-polymer		306.2	1	Chemie (2DT)

Sequence modifications

A: 30 - 1254 (UniProt: P15801)

PDB	External Database	Details
Met 28	-	expression tag
Ala 29	-	expression tag
Val 222	Ile 222	conflict
Lys 357	Glu 357	conflict
Ala 420	Val 420	conflict
Met 540	Thr 540	conflict
Asn 541	His 541	conflict
Asn 616	Ser 616	conflict
Thr 661	Ala 661	conflict
Pro 978	Ser 978	conflict
Ser 986	Asn 986	conflict
Lys 1255	-	expression tag
Leu 1256	-	expression tag
Ala 1257	-	expression tag
Ala 1258	-	expression tag
Ala 1259	-	expression tag
Leu 1260	-	expression tag
Glu 1261	-	expression tag
His 1262	-	expression tag
His 1263	-	expression tag
His 1264	-	expression tag
His 1265	-	expression tag
His 1266	-	expression tag
His 1267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168850.8
Non-Polymers*	Number of molecules	3
	Total formula weight	821.7
All*	Total formula weight	169672.5

*Water molecules are not included.