9D0Y

Map of endoH-treated hemagglutinin A/Sing/INFIMH/16

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, B, C)	Hemagglutinin HA1 chain	polymer	340	37598.8	3	UniProt (A0A2R4U332) Pfam (PF00509)	Influenza A virus
2	B, D, F (D, E, F)	Hemagglutinin HA2 chain, Green fluorescent protein fusion	polymer	486	55162.6	3	UniProt (Q38SQ8) UniProt (P42212) Pfam (PF00509) Pfam (PF01353)	Influenza A virus
3	AA, BA, CA, DA, EA... (C, A, F, D, B...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	30	Chemie (NAG)

Sequence modifications

A, B, C: 11 - 326 (UniProt: A0A2R4U332)

PDB	External Database	Details
Met -13	-	initiating methionine
Pro -12	-	expression tag
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Leu -8	-	expression tag
Gln -7	-	expression tag
Pro -6	-	expression tag
Leu -5	-	expression tag
Ala -4	-	expression tag
Thr -3	-	expression tag
Leu -2	-	expression tag
Tyr -1	-	expression tag
Leu 0	-	expression tag
Leu 1	-	expression tag
Gly 2	-	expression tag
Met 3	-	expression tag
Leu 4	-	expression tag
Val 5	-	expression tag
Ala 6	-	expression tag
Ser 7	-	expression tag
Val 8	-	expression tag
Leu 9	-	expression tag
Ala 10	-	expression tag

D, E, F: -2 - 174 (UniProt: Q38SQ8)

PDB	External Database	Details
Arg 32	Thr 377	conflict
Ile 45	Val 390	conflict
Gly 57	Glu 402	conflict
Val 77	Ile 422	conflict
Lys 123	Arg 468	conflict
Glu 150	Gly 495	conflict
Glu 155	Gly 500	conflict
Asn 160	Asp 505	conflict
Arg 175	-	linker
Met 176	-	linker
Lys 177	-	linker
Gln 178	-	linker
Ile 179	-	linker
Glu 180	-	linker
Asp 181	-	linker
Lys 182	-	linker
Ile 183	-	linker
Glu 184	-	linker
Glu 185	-	linker
Ile 186	-	linker
Glu 187	-	linker
Ser 188	-	linker
Lys 189	-	linker
Gln 190	-	linker
Lys 191	-	linker
Lys 192	-	linker
Ile 193	-	linker
Glu 194	-	linker
Asn 195	-	linker
Glu 196	-	linker
Ile 197	-	linker
Ala 198	-	linker
Arg 199	-	linker
Ile 200	-	linker
Lys 201	-	linker
Lys 202	-	linker
Ile 203	-	linker
Lys 204	-	linker
Leu 205	-	linker
Val 206	-	linker
Pro 207	-	linker
Arg 208	-	linker
Gly 209	-	linker
Ser 210	-	linker
Val 211	-	linker
Asp 212	-	linker
Glu 213	-	linker
Asn 214	-	linker
Leu 215	-	linker
Tyr 216	-	linker
Phe 217	-	linker
Gln 218	-	linker
Ala 219	-	linker

D, E, F: 220 - 452 (UniProt: P42212)

PDB	External Database	Details
Arg 249	Ser 30	conflict
Asn 258	Tyr 39	conflict
Leu 283	Phe 64	conflict
Thr 284	Ser 65	conflict
Arg 299	Gln 80	conflict
Ser 318	Phe 99	conflict
Thr 324	Asn 105	conflict
Phe 364	Tyr 145	conflict
Thr 372	Met 153	conflict
Ala 382	Val 163	conflict
Val 390	Ile 171	conflict
Val 425	Ala 206	conflict
Ser 453	-	expression tag
Ser 454	-	expression tag
Ala 455	-	expression tag
Trp 456	-	expression tag
Ser 457	-	expression tag
His 458	-	expression tag
Pro 459	-	expression tag
Gln 460	-	expression tag
Phe 461	-	expression tag
Glu 462	-	expression tag
Lys 463	-	expression tag
Gly 464	-	expression tag
Gly 465	-	expression tag
Gly 466	-	expression tag
Ser 467	-	expression tag
Gly 468	-	expression tag
Gly 469	-	expression tag
Gly 470	-	expression tag
Ser 471	-	expression tag
Gly 472	-	expression tag
Gly 473	-	expression tag
Ser 474	-	expression tag
Ala 475	-	expression tag
Trp 476	-	expression tag
Ser 477	-	expression tag
His 478	-	expression tag
Pro 479	-	expression tag
Gln 480	-	expression tag
Phe 481	-	expression tag
Glu 482	-	expression tag
Lys 483	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	278284.3
Non-Polymers*	Number of molecules	30
	Total formula weight	6636.2
All*	Total formula weight	284920.6

*Water molecules are not included.