9D09

Crystal structure of IgG1 FC M252H at pH 5.6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Immunoglobulin gamma-1 heavy chain Fc Fragment	polymer	231	25971.4	2	UniProt (P0DOX5)	Homo sapiens (human)	Immunoglobulin gamma-1 heavy chain NIE
2	C (C)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1219.1	1	In PDB GlyTouCan (G32156ZV)

Sequence modifications

A, B: 236 - 447 (UniProt: P0DOX5)

PDB	External Database	Details
Met 217	-	initiating methionine
Gly 218	-	expression tag
Trp 219	-	expression tag
Ser 220	-	expression tag
Cys 221	-	expression tag
Ile 222	-	expression tag
Ile 223	-	expression tag
Leu 224	-	expression tag
Phe 225	-	expression tag
Leu 226	-	expression tag
Val 227	-	expression tag
Ala 228	-	expression tag
Thr 229	-	expression tag
Ala 230	-	expression tag
Thr 231	-	expression tag
Gly 232	-	expression tag
Val 233	-	expression tag
His 234	-	expression tag
Ser 235	-	expression tag
His 252	Met 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51942.8
Branched	Number of molecules	1
	Total formula weight	1219.1
All*	Total formula weight	53161.9

*Water molecules are not included.