9CYJ

Cryo-EM structure of FNI9 IgG in complex with influenza virus neuraminidase from A/Kansas/14/2017 (H3N2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, E, F (C, B, A, D)	Neuraminidase	polymer	469	52145.5	4	UniProt (A0A3G8EZM0)	Influenza A virus
2	B (H)	FNI9 IgG heavy chain	polymer	231	24418.1	1		Homo sapiens
3	C (L)	FNI9 IgG light chain	polymer	215	23565.1	1		Homo sapiens
4	G, K, S (E, J, S)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	3	In PDB GlyTouCan (G86851RC)
5	H, J, L, N, O... (F, I, K, N, O...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
6	I, M, P, U (G, M, P, U)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	4	In PDB GlyTouCan (G80966KZ)
7	R (R)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
8	CA, FA, W, Z (A, D, C, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
9	AA, BA, DA, EA, GA... (B, A, D, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)

Sequence modifications

C, B, A, D: 83 - 469 (UniProt: A0A3G8EZM0)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 2	-	expression tag
Ala 3	-	expression tag
Met 4	-	expression tag
Lys 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Leu 8	-	expression tag
Cys 9	-	expression tag
Cys 10	-	expression tag
Val 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Leu 14	-	expression tag
Cys 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Val 18	-	expression tag
Phe 19	-	expression tag
Val 20	-	expression tag
Ser 21	-	expression tag
Pro 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
Gly 29	-	expression tag
Ser 30	-	expression tag
Ser 31	-	expression tag
Ser 32	-	expression tag
Ser 33	-	expression tag
Asp 34	-	expression tag
Tyr 35	-	expression tag
Ser 36	-	expression tag
Asp 37	-	expression tag
Leu 38	-	expression tag
Gln 39	-	expression tag
Arg 40	-	expression tag
Val 41	-	expression tag
Lys 42	-	expression tag
Gln 43	-	expression tag
Glu 44	-	expression tag
Leu 45	-	expression tag
Leu 46	-	expression tag
Glu 47	-	expression tag
Glu 48	-	expression tag
Val 49	-	expression tag
Lys 50	-	expression tag
Lys 51	-	expression tag
Glu 52	-	expression tag
Leu 53	-	expression tag
Gln 54	-	expression tag
Lys 55	-	expression tag
Val 56	-	expression tag
Lys 57	-	expression tag
Glu 58	-	expression tag
Glu 59	-	expression tag
Ile 60	-	expression tag
Ile 61	-	expression tag
Glu 62	-	expression tag
Ala 63	-	expression tag
Phe 64	-	expression tag
Val 65	-	expression tag
Gln 66	-	expression tag
Glu 67	-	expression tag
Leu 68	-	expression tag
Arg 69	-	expression tag
Lys 70	-	expression tag
Arg 71	-	expression tag
Gly 72	-	expression tag
Ser 73	-	expression tag
Glu 74	-	expression tag
Asn 75	-	expression tag
Leu 76	-	expression tag
Tyr 77	-	expression tag
Phe 78	-	expression tag
Gln 79	-	expression tag
Ser 80	-	expression tag
Gly 81	-	expression tag
Ser 82	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	256565.2
Branched	Number of molecules	16
	Total formula weight	10656.8
Non-Polymers*	Number of molecules	12
	Total formula weight	1930.0
All*	Total formula weight	269151.9

*Water molecules are not included.