9CYH

Cryo-EM structure of DA03E17 Fab in complex with influenza virus neuraminidase from B/Colorado/06/2017

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (H, E, F, G)	DA03E17 Fab heavy chain	polymer	231	24554.4	4		Homo sapiens
2	B, E, H, K (L, I, J, K)	DA03E17 Fab light chain	polymer	215	23486.0	4		Homo sapiens
3	C, F, I, L (A, B, C, D)	Neuraminidase	polymer	482	53536.7	4	UniProt (A0A1X9RX69)	Influenza B virus (B/Colorado/06/2017)
4	M, N, O, P, Q... (M, N, O, P, Q...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
5	U, V, W, X (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)

Sequence modifications

A, B, C, D: 76 - 466 (UniProt: A0A1X9RX69)

PDB	External Database	Details
Met -15	-	initiating methionine
Tyr -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Gln -11	-	expression tag
Leu -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Cys -7	-	expression tag
Val -6	-	expression tag
Thr -5	-	expression tag
Leu -4	-	expression tag
Thr -3	-	expression tag
Leu -2	-	expression tag
Val -1	-	expression tag
Leu 0	-	expression tag
Leu 1	-	expression tag
Val 2	-	expression tag
Asn 3	-	expression tag
Ser 4	-	expression tag
Gln 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Trp 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Pro 18	-	expression tag
Gln 19	-	expression tag
Phe 20	-	expression tag
Glu 21	-	expression tag
Lys 22	-	expression tag
Gly 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
Ser 26	-	expression tag
Ser 27	-	expression tag
Asp 28	-	expression tag
Tyr 29	-	expression tag
Ser 30	-	expression tag
Asp 31	-	expression tag
Leu 32	-	expression tag
Gln 33	-	expression tag
Arg 34	-	expression tag
Val 35	-	expression tag
Lys 36	-	expression tag
Gln 37	-	expression tag
Glu 38	-	expression tag
Leu 39	-	expression tag
Leu 40	-	expression tag
Glu 41	-	expression tag
Glu 42	-	expression tag
Val 43	-	expression tag
Lys 44	-	expression tag
Lys 45	-	expression tag
Glu 46	-	expression tag
Leu 47	-	expression tag
Gln 48	-	expression tag
Lys 49	-	expression tag
Val 50	-	expression tag
Lys 51	-	expression tag
Glu 52	-	expression tag
Glu 53	-	expression tag
Ile 54	-	expression tag
Ile 55	-	expression tag
Glu 56	-	expression tag
Ala 57	-	expression tag
Phe 58	-	expression tag
Val 59	-	expression tag
Gln 60	-	expression tag
Glu 61	-	expression tag
Leu 62	-	expression tag
Arg 63	-	expression tag
Lys 64	-	expression tag
Arg 65	-	expression tag
Gly 66	-	expression tag
Ser 67	-	expression tag
Leu 68	-	expression tag
Val 69	-	expression tag
Pro 70	-	expression tag
Arg 71	-	expression tag
Gly 72	-	expression tag
Ser 73	-	expression tag
Gly 74	-	expression tag
Gly 75	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	406308.5
Branched	Number of molecules	8
	Total formula weight	3395.2
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	409864.0

*Water molecules are not included.