9CYG

Cryo-EM structure of influenza virus neuraminidase from A/Kansas/14/2017 (H3N2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Neuraminidase	polymer	469	52145.5	4	UniProt (A0A3G8EZM0) UniProt (by SIFTS) (A0A2S0X6Y5)	Influenza A virus
2	E, I, M, Q (E, I, M, Q)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	4	In PDB GlyTouCan (G86851RC)
3	F, H, J, L, N... (F, H, J, L, N...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
4	G, K, O, S (G, K, O, S)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	4	In PDB GlyTouCan (G80966KZ)
5	AA, DA, U, X (C, D, A, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	BA, CA, EA, FA, V... (C, D, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)

Sequence modifications

A, B, C, D: 38 - 469 (UniProt: A0A3G8EZM0)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 2	-	expression tag
Ala 3	-	expression tag
Met 4	-	expression tag
Lys 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Leu 8	-	expression tag
Cys 9	-	expression tag
Cys 10	-	expression tag
Val 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Leu 14	-	expression tag
Cys 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Val 18	-	expression tag
Phe 19	-	expression tag
Val 20	-	expression tag
Ser 21	-	expression tag
Pro 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
Gly 29	-	expression tag
Ser 30	-	expression tag
Ser 31	-	expression tag
Ser 32	-	expression tag
Ser 33	-	expression tag
Asp 34	-	expression tag
Tyr 35	-	expression tag
Ser 36	-	expression tag
Asp 37	-	expression tag
Gln 39	His 36	conflict
Arg 40	Phe 37	conflict
Val 41	Lys 38	conflict
Lys 42	Gln 39	conflict
Gln 43	Tyr 40	conflict
Leu 45	Phe 42	conflict
Leu 46	Asn 43	conflict
Glu 47	Pro 44	conflict
Glu 48	Pro 45	conflict
Val 49	Pro 46	conflict
Lys 50	Asn 47	conflict
Lys 51	Asn 48	conflict
Glu 52	Gln 49	conflict
Leu 53	Val 50	conflict
Gln 54	Met 51	conflict
Lys 55	Unk 52	conflict
Val 56	Cys 53	conflict
Lys 57	Glu 54	conflict
Glu 58	Pro 55	conflict
Glu 59	Thr 56	conflict
Ala 63	Arg 60	conflict
Phe 64	Asn 61	conflict
Val 65	Ile 62	conflict
Gln 66	Thr 63	conflict
-	Ile 65	deletion
-	Val 66	deletion
-	Tyr 67	deletion
Arg 69	Thr 69	conflict
Lys 70	Asn 70	conflict
Arg 71	Thr 71	conflict
Gly 72	Thr 72	conflict
Ser 73	Ile 73	conflict
Asn 75	Arg 75	conflict
Leu 76	Glu 76	conflict
Tyr 77	Ile 77	conflict
Phe 78	Cys 78	conflict
Gln 79	Pro 79	conflict
Ser 80	Lys 80	conflict
Gly 81	Pro 81	conflict
Ser 82	Ala 82	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	208582.1
Branched	Number of molecules	16
	Total formula weight	10453.6
Non-Polymers*	Number of molecules	12
	Total formula weight	1930.0
All*	Total formula weight	220965.6

*Water molecules are not included.