9CSL

Structure of mutant human geranylgeranyl pyrophosphate synthase (Y246D-C247L) in complex with isopentenyl pyrophosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Geranylgeranyl pyrophosphate synthase	polymer	322	37436.5	2	UniProt (O95749) Pfam (PF00348)	Homo sapiens (human)	GGPP synthase,GGPPSase,(2E,6E)-farnesyl diphosphate synthase,Dimethylallyltranstransferase,Farnesyl diphosphate synthase,Farnesyltranstransferase,Geranylgeranyl diphosphate synthase,Geranyltranstransferase
2	C (A)	3-METHYLBUT-3-ENYL TRIHYDROGEN DIPHOSPHATE	non-polymer		246.1	1	Chemie (IPE)
3	D (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	E, F, G, H, I... (A, B)	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
5	Q, R (A, B)	water	water		18.0	424	Chemie (HOH)

Sequence modifications

A, B: 1 - 300 (UniProt: O95749)

PDB	External Database	Details
Met -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Asp 246	Tyr 246	engineered mutation
Leu 247	Cys 247	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74873.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1434.3
All*	Total formula weight	76307.3

*Water molecules are not included.