9CM5

CryoEM Strucuture of TcdB in complex with De Novo Minibinder fzd48

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	De Novo Minibinder fzd48	polymer	76	8926.1	1		synthetic construct
2	B (A)	Toxin B	polymer	2119	241229.9	1	UniProt (P18177)	Clostridioides difficile

Sequence modifications

A: 1 - 2100 (UniProt: P18177)

PDB	External Database	Details
Met -1	-	initiating methionine
Tyr 0	-	expression tag
Ala 102	Trp 102	conflict
Asn 286	Asp 286	conflict
Asn 288	Asp 288	conflict
Ala 543	Leu 543	conflict
Gly 2101	-	expression tag
Tyr 2102	-	expression tag
Arg 2103	-	expression tag
Pro 2104	-	expression tag
His 2105	-	expression tag
Ala 2106	-	expression tag
Gly 2107	-	expression tag
Leu 2108	-	expression tag
Arg 2109	-	expression tag
Gly 2110	-	expression tag
Ser 2111	-	expression tag
His 2112	-	expression tag
His 2113	-	expression tag
His 2114	-	expression tag
His 2115	-	expression tag
His 2116	-	expression tag
His 2117	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	250156.0
All*	Total formula weight	250156.0

*Water molecules are not included.