9CJJ
Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | guide RNA (25-MER) | polymer | 42 | 13419.0 | 1 | synthetic construct | ||
| 2 | B (A) | CRISPR-associated endonuclease Cas12a | polymer | 1310 | 151680.3 | 1 | UniProt (U2UMQ6) Pfam (PF18501) Pfam (PF21918) Pfam (PF18516) Pfam (PF18510) | Acidaminococcus sp. BV3L6 | AsCpf1,CRISPR-associated endonuclease Cpf1 |
| 3 | C (C) | Non-target DNA strand | polymer | 31 | 9567.2 | 1 | synthetic construct | ||
| 4 | D (D) | Target DNA strand | polymer | 31 | 9557.2 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1307 (UniProt: U2UMQ6)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Cys 551 | Asn 551 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 184223.6 | |
| All* | Total formula weight | 184223.6 |
