9CIT

Crystal structure of pyrophosphate-dependent phosphofructokinase from Candidatus Prometheoarchaeum syntrophicum with phosphoenolpyruvate, phosphate and Mg2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (B, A, C, D)	6-phosphofructokinase	polymer	424	46779.3	4	UniProt (A0A5B9D762) Pfam (PF00365)	Candidatus Prometheoarchaeum syntrophicum
2	E, H (B, A)	PHOSPHOENOLPYRUVATE	non-polymer		168.0	2	Chemie (PEP)
3	F, I, J, M (B, A, D)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
4	G, K, L (A, C, D)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	N, O, P, Q (B, A, C, D)	water	water		18.0	27	Chemie (HOH)

Sequence modifications

B, A, C, D: 3 - 406 (UniProt: A0A5B9D762)

PDB	External Database	Details
Met -17	-	initiating methionine
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Pro -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	187117.2
Non-Polymers*	Number of molecules	9
	Total formula weight	788.9
All*	Total formula weight	187906.0

*Water molecules are not included.