9CGK

CryoEM structure of delta opioid receptor bound to G proteins and a full bitopic agonist

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Delta-type opioid receptor	polymer	303	33663.4	1	UniProt (P41143) Pfam (PF00001)	Homo sapiens (human)	D-OR-1,DOR-1
2	B (E)	ScFv16 protein	polymer	251	26679.7	1		Mus musculus (mouse)
3	C (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37342.8	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (B)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
6	F (A)	N-{5-[(4bS,8R,8aS,15bR)-1,8a-dihydroxy-5,6,8,8a,9,15b-hexahydro-7H-4,8-methano[1]benzofuro[3,2-c]pyrido[3,4-b]acridin-7-yl]pentyl}guanidine	non-polymer		499.6	1	Chemie (A1AWD)

Sequence modifications

A: 38 - 338 (UniProt: P41143)

PDB	External Database	Details
Gly 36	-	expression tag
Ser 37	-	expression tag

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 1	-	expression tag

B: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	145961.1
Non-Polymers*	Number of molecules	1
	Total formula weight	499.6
All*	Total formula weight	146460.7

*Water molecules are not included.