9CGJ

CryoEM structure of delta opioid receptor bound to G proteins and a partial agonist

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Delta-type opioid receptor	polymer	303	33663.4	1	UniProt (P41143) Pfam (PF00001)	Homo sapiens (human)	D-OR-1,DOR-1
2	B (E)	ScFv16 protein	polymer	251	26679.7	1		Mus musculus (mouse)
3	C (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (B)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37342.8	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
6	F (A)	N-{6-[(4bS,8R,8aS,15bR)-1,8a-dihydroxy-5,6,8,8a,9,15b-hexahydro-7H-4,8-methano[1]benzofuro[3,2-c]pyrido[3,4-b]acridin-7-yl]hexyl}guanidine	non-polymer		513.6	1	Chemie (A1AWC)

Sequence modifications

A: 38 - 338 (UniProt: P41143)

PDB	External Database	Details
Gly 36	-	expression tag
Ser 37	-	expression tag

B: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	145961.1
Non-Polymers*	Number of molecules	1
	Total formula weight	513.6
All*	Total formula weight	146474.7

*Water molecules are not included.