9CG9

Cryo-EM structure of an HMGB1 box bound to nucleosome at SHL-2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15271.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	Widom 601 DNA reverse strand (147-mer)	polymer	154	47746.4	1		synthetic construct
6	J (J)	Widom 601 DNA forward strand (147-mer)	polymer	154	47328.1	1		synthetic construct
7	K (K)	High mobility group protein B1	polymer	217	25092.0	1	UniProt (P09429) Pfam (PF09011) Pfam (PF00505)	Homo sapiens (human)	High mobility group protein 1,HMG-1

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation
Ala 110	Cys 111	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Thr 32	Ser 33	engineered mutation

K: 0 - 214 (UniProt: P09429)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	228242.8
All*	Total formula weight	228242.8

*Water molecules are not included.