9C7C

Diheteromeric GluN1/GluN2A (delM653) in nanodisc complexed with glycine, glutamate, and GNE-4123, open conformation

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Glutamate receptor ionotropic, NMDA 1,Green fluorescent protein	polymer	1108	124759.2	2	UniProt (P35439) UniProt (P42212) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) Pfam (PF01353)	Rattus norvegicus (Norway rat)	GluN1,Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1,NMD-R1
2	B, D (B, D)	Glutamate receptor ionotropic, NMDA 2A,Green fluorescent protein chimera	polymer	1126	126356.2	2	UniProt (Q00959) UniProt (P42212) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) Pfam (PF01353)	Rattus norvegicus (Norway rat)	GluN2A,Glutamate [NMDA] receptor subunit epsilon-1,N-methyl D-aspartate receptor subtype 2A,NMDAR2A,NR2A
3	E, F, G, H, I... (E, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	11	In PDB GlyTouCan (G42666HT)
4	LA, P (C, A)	4-cyclohexyl-N-[(8R)-2-cyclopropyl-7-hydroxy-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-6-yl]benzene-1-sulfonamide	non-polymer		427.5	2	Chemie (A1AUV)
5	AA, AB, BA, CA, DA... (B, D, C, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	24	Chemie (NAG)
6	X (A)	HEPTANE	non-polymer		100.2	1	Chemie (HP6)
7	BB, CB, FA, FB, GA... (D, B, C, A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	8	Chemie (Y01)
8	UA, Z (C, A)	GLYCINE	non-polymer		75.1	2	Chemie (GLY)
9	DB, HA (D, B)	DODECANE	non-polymer		170.3	2	Chemie (D12)
10	GB, JA (D, B)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
11	KA, SA (B, C)	DECANE	non-polymer		142.3	2	Chemie (D10)
12	EB (D)	HEXANE	non-polymer		86.2	1	Chemie (HEX)

Sequence modifications

A, C: 1 - 847 (UniProt: P35439)

PDB	External Database	Details
Leu 848	-	linker
Val 849	-	linker
Pro 850	-	linker
Arg 851	-	linker
Gly 852	-	linker
Ser 853	-	linker
Ala 854	-	linker
Ala 855	-	linker
Ala 856	-	linker
Ala 857	-	linker
Val 858	-	linker
Ser 859	-	linker
Lys 860	-	linker
Gly 861	-	linker

A, C: 862 - 1095 (UniProt: P42212)

PDB	External Database	Details
Leu 921	Phe 64	conflict
Thr 922	Ser 65	conflict
Lys 1063	Ala 206	conflict
Leu 1088	His 231	conflict
Ser 1096	-	expression tag
Gly 1097	-	expression tag
Leu 1098	-	expression tag
Arg 1099	-	expression tag
Ser 1100	-	expression tag
His 1101	-	expression tag
His 1102	-	expression tag
His 1103	-	expression tag
His 1104	-	expression tag
His 1105	-	expression tag
His 1106	-	expression tag
His 1107	-	expression tag
His 1108	-	expression tag

B, D: 1 - 864 (UniProt: Q00959)

PDB	External Database	Details
-	Met 653	deletion
Thr 757	Ser 758	conflict
Gly 865	-	linker
Leu 866	-	linker
Val 867	-	linker
Pro 868	-	linker
Arg 869	-	linker
Gly 870	-	linker
Ser 871	-	linker
Ala 872	-	linker
Ala 873	-	linker
Ala 874	-	linker
Ala 875	-	linker
Val 876	-	linker

B, D: 877 - 1113 (UniProt: P42212)

PDB	External Database	Details
Leu 939	Phe 64	conflict
Thr 940	Ser 65	conflict
Lys 1081	Ala 206	conflict
Leu 1106	His 231	conflict
Ser 1114	-	expression tag
Gly 1115	-	expression tag
Leu 1116	-	expression tag
Arg 1117	-	expression tag
Ser 1118	-	expression tag
Trp 1119	-	expression tag
Ser 1120	-	expression tag
His 1121	-	expression tag
Pro 1122	-	expression tag
Gln 1123	-	expression tag
Phe 1124	-	expression tag
Glu 1125	-	expression tag
Lys 1126	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	502230.8
Branched	Number of molecules	11
	Total formula weight	4668.4
Non-Polymers*	Number of molecules	44
	Total formula weight	11313.8
All*	Total formula weight	518213.1

*Water molecules are not included.