9BUO

CryoEM structure of DPOR in the presence of ADP-AlF3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H (G, H, E, F)	Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein	polymer	318	34850.3	4	UniProt (Q9RFD6) Pfam (PF00142)	Cereibacter sphaeroides	LI-POR subunit L
2	C, E (A, C)	Light-independent protochlorophyllide reductase subunit N	polymer	428	46188.8	2	UniProt (B9KK24) Pfam (PF00148)	Cereibacter sphaeroides	DPOR subunit N,LI-POR subunit N
3	D, F (B, D)	Light-independent protochlorophyllide reductase subunit B	polymer	534	58302.2	2	UniProt (Q9Z5D9) Pfam (PF00148) Pfam (PF08369)	Cereibacter sphaeroides	DPOR subunit B,LI-POR subunit B
4	I, J, M, P (H, A, C, E)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
5	K, N (A, C)	Protochlorophyllide	non-polymer		613.0	2	Chemie (PMR)
6	L, O (B, D)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)

Sequence modifications

G, H, E, F: 1 - 297 (UniProt: Q9RFD6)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Gln -9	-	expression tag
Asp -8	-	expression tag
Pro -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Glu 289	Asp 289	variant

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	348383.4
Non-Polymers*	Number of molecules	8
	Total formula weight	2759.6
All*	Total formula weight	351142.9

*Water molecules are not included.