9BTV
Cryo-EM structure of rhesus antibody T646-a.01 in complex with HIV-1 Env trimer Q23.17 MD39
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, D (A, C, E) | Envelope glycoprotein gp120 | polymer | 469 | 52996.1 | 3 | UniProt (O55774) Pfam (PF00516) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, E, F (B, F, G) | Q23.MD39 Transmembrane protein gp41 | polymer | 153 | 17313.6 | 3 | UniProt (O55774) Pfam (PF00517) | Human immunodeficiency virus 1 | |
| 3 | G (H) | T646-a.01 heavy chain | polymer | 245 | 26645.6 | 1 | Macaca mulatta (Rhesus monkey) | ||
| 4 | H (L) | T646-a.01 light chain | polymer | 216 | 22689.9 | 1 | Macaca mulatta (Rhesus monkey) | ||
| 5 | I (D) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 6 | J (I) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 7 | DA, GA, K, L, MA... (d, g, J, K, m...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 12 | In PDB GlyTouCan (G15407YE) | |||
| 8 | AA, BA, CA, EA, FA... (a, b, c, e, f...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 24 | In PDB GlyTouCan (G42666HT) | |||
| 9 | IA, KA (i, k) | alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G22573RC) | |||
| 10 | JA (j) | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G34442SS) | |||
| 11 | AB, BB, CB, DB, EB... (A, B, C, E, F...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 19 | Chemie (NAG) |
Sequence modifications
A, C, E: 31 - 508 (UniProt: O55774)
B, F, G: 512 - 664 (UniProt: O55774)
| PDB | External Database | Details |
|---|---|---|
| Glu 106 | Thr 105 | conflict |
| Ile 271 | Thr 263 | conflict |
| Val 304 | Arg 296 | conflict |
| Tyr 319 | Ala 309 | conflict |
| Ser 473 | Gly 463 | conflict |
| Cys 501 | Ala 491 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 519 | Phe 509 | engineered mutation |
| Ala 533 | Thr 523 | engineered mutation |
| Asn 543 | Gln 533 | conflict |
| Pro 559 | Ile 549 | engineered mutation |
| Pro 561 | Ala 551 | engineered mutation |
| His 570 | Val 560 | engineered mutation |
| His 585 | Arg 575 | engineered mutation |
| Cys 605 | Thr 595 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 260264.7 | |
| Branched | Number of molecules | 41 |
| Total formula weight | 21454.0 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 4203.0 | |
| All* | Total formula weight | 285921.6 |
