9BSB

Global reconstruction of DRD2 bound to LSD in complex with a mini-GoA and scFv16 obtained by cryo-electron microscopy (cryoEM)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	348	38146.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (R)	Soluble cytochrome b562,D(2) dopamine receptor	polymer	588	67234.6	1	UniProt (P0ABE7) UniProt (P14416) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,Dopamine D2 receptor
4	D (E)	Single chain Fab 16 (scFv16)	polymer	268	28674.9	1		Mus musculus
5	E (A)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40221.6	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
6	F (R)	(8alpha)-N,N-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide	non-polymer		323.4	1	Chemie (7LD)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag

R: -116 - -12 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -144	-	expression tag
Tyr -143	-	expression tag
Lys -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Asp -139	-	expression tag
Asp -138	-	expression tag
Ala -137	-	expression tag
Lys -136	-	expression tag
Leu -135	-	expression tag
Gln -134	-	expression tag
Thr -133	-	expression tag
Met -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
Trp -110	Met 29	conflict
Ile -15	His 124	conflict
Leu -11	-	linker
Ala -10	-	linker
Ser -9	-	linker
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Thr 0	-	linker

R: 1 - 443 (UniProt: P14416)
A: 1 - 354 (UniProt: P09471)

PDB	External Database	Details
Asp 9	Glu 9	conflict
Lys 10	Arg 10	conflict
Val 13	Leu 13	conflict
Met 18	Ala 18	conflict
Asp 42	Gly 42	conflict
Asn 43	Glu 43	conflict
Asp 227	Ala 227	conflict
Asp 230	Gly 230	conflict
Ala 332	Ile 332	conflict
Ile 335	Val 335	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	182138.9
Non-Polymers*	Number of molecules	1
	Total formula weight	323.4
All*	Total formula weight	182462.3

*Water molecules are not included.