9BS6
CryoEM structure of ThermoCas9 in post-cleavage state with a DNA containing NNNNCGA PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA (25-MER) | polymer | 26 | 7883.1 | 1 | synthetic construct | ||
| 2 | B (D) | DNA (5'-D(P*GP*TP*AP*TP*AP*CP*AP*CP*GP*AP*AP*GP*CP*T)-3') | polymer | 34 | 10572.8 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*AP*GP*CP*TP*TP*CP*GP*TP*GP*TP*AP*TP*AP*C)-3') | polymer | 14 | 4270.8 | 1 | synthetic construct | ||
| 4 | D (X) | DNA (5'-D(P*CP*TP*AP*GP*A)-3') | polymer | 6 | 1808.2 | 1 | synthetic construct | ||
| 5 | E (A) | CRISPR-associated endonuclease Cas9 | polymer | 1082 | 126563.5 | 1 | UniProt (A0A1W6VMQ3) Pfam (PF18470) Pfam (PF13395) Pfam (PF18541) UniProt (by SIFTS) (A0A4R1QIZ8) | Geobacillus thermodenitrificans | |
| 6 | F (B) | RNA (114-MER) | polymer | 149 | 47798.1 | 1 | Geobacillus thermodenitrificans | ||
| 7 | G (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 8 | H, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 9 | J, K (A, B) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 198896.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 571.8 | |
| All* | Total formula weight | 199468.3 |
