9BPU

Structure of the IFN-lambda4/IFN-lambdaR1/IL-10Rbeta receptor complex with an engineered IL-10Rbeta

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Interferon lambda-4	polymer	175	19219.2	1	UniProt (K9M1U5) Pfam (PF15177)	Homo sapiens (human)	IFN-lambda-4
2	B (A)	Interleukin-10 receptor subunit beta	polymer	212	24773.6	1	UniProt (Q08334) Pfam (PF01108) Pfam (PF09294)	Homo sapiens (human)	IL-10 receptor subunit beta,IL-10R subunit beta,IL-10RB,Cytokine receptor class-II member 4,Cytokine receptor family 2 member 4,CRF2-4,Interleukin-10 receptor subunit 2,IL-10R subunit 2,IL-10R2
3	C (B)	Interferon lambda receptor 1	polymer	209	23628.1	1	UniProt (Q8IU57) Pfam (PF01108)	Homo sapiens (human)	IFN-lambda receptor 1,IFN-lambda-R1,Cytokine receptor class-II member 12,Cytokine receptor family 2 member 12,CRF2-12,Interleukin-28 receptor subunit alpha,IL-28 receptor subunit alpha,IL-28R-alpha,IL-28RA,Likely interleukin or cytokine receptor 2,LICR2
4	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	E (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)

Sequence modifications

C: 21 - 179 (UniProt: K9M1U5)

PDB	External Database	Details
Gly 16	-	expression tag
Gly 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
Gly 20	-	expression tag
Ala 180	-	expression tag
Ala 181	-	expression tag
Ala 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag

A: 20 - 220 (UniProt: Q08334)

PDB	External Database	Details
Gly 18	-	expression tag
Ser 19	-	expression tag
Gln 49	Asn 49	conflict
Gln 68	Asn 68	conflict
Gln 102	Asn 102	conflict
Asp 147	Asn 147	conflict
Met 148	Val 148	conflict
Gln 161	Asn 161	conflict
Arg 184	Trp 184	conflict
Ala 221	-	expression tag
Ala 222	-	expression tag
Ala 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag

B: 1 - 206 (UniProt: Q8IU57)

PDB	External Database	Details
Ala 207	-	expression tag
Ala 208	-	expression tag
Ala 209	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67620.9
Branched	Number of molecules	2
	Total formula weight	1010.9
All*	Total formula weight	68631.8

*Water molecules are not included.