9BOI
Cryo-EM structure of human Spns1 in complex with LPC (18:1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | mVenus,Maltose/maltodextrin-binding periplasmic protein,Protein spinster homolog 1,Designed ankyrin repeat protein (DARPin) | polymer | 1275 | 139299.7 | 1 | UniProt (P42212) UniProt (P0AEX9) UniProt (Q9H2V7) Pfam (PF01547) Pfam (PF07690) | Aequorea victoria | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,HSpin1,SPNS1,Spinster-like protein 1 |
| 2 | B (A) | (4R,7R,18Z)-4,7-dihydroxy-N,N,N-trimethyl-10-oxo-3,5,9-trioxa-4-phosphaheptacos-18-en-1-aminium 4-oxide | non-polymer | 522.7 | 1 | Chemie (42H) |
Sequence modifications
A: -566 - -330 (UniProt: P42212)
A: -321 - 41 (UniProt: P0AEX9)
A: 56 - 528 (UniProt: Q9H2V7)
A: 539 - 706 (PDB: 9BOI)
| PDB | External Database | Details |
|---|---|---|
| Met -568 | - | initiating methionine |
| Val -567 | - | expression tag |
| Leu -522 | Phe 46 | conflict |
| Leu -504 | Phe 64 | conflict |
| Gly -503 | Ser 65 | conflict |
| Leu -500 | Val 68 | conflict |
| Ala -496 | Ser 72 | conflict |
| Thr -415 | Met 153 | conflict |
| Ala -405 | Val 163 | conflict |
| Gly -393 | Ser 175 | conflict |
| Tyr -365 | Thr 203 | conflict |
| Lys -362 | Ala 206 | conflict |
| Leu -337 | His 231 | conflict |
| Leu -329 | - | linker |
| Glu -328 | - | linker |
| Val -327 | - | linker |
| Leu -326 | - | linker |
| Phe -325 | - | linker |
| Gln -324 | - | linker |
| Gly -323 | - | linker |
| Pro -322 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ile -128 | Val 222 | conflict |
| Val -12 | Ala 338 | conflict |
| Val -7 | Ile 343 | conflict |
| Ala 35 | Glu 385 | conflict |
| Ala 38 | Lys 388 | conflict |
| Ala 39 | Asp 389 | conflict |
| Ala 42 | - | linker |
| Glu 43 | - | linker |
| Glu 44 | - | linker |
| Glu 45 | - | linker |
| Lys 46 | - | linker |
| Arg 47 | - | linker |
| Lys 48 | - | linker |
| Ala 49 | - | linker |
| Glu 50 | - | linker |
| Glu 51 | - | linker |
| Glu 52 | - | linker |
| Lys 53 | - | linker |
| Arg 54 | - | linker |
| Lys 55 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 529 | - | linker |
| Gly 530 | - | linker |
| Gly 531 | - | linker |
| Gly 532 | - | linker |
| Ser 533 | - | linker |
| Gly 534 | - | linker |
| Gly 535 | - | linker |
| Gly 536 | - | linker |
| Gly 537 | - | linker |
| Ser 538 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 139299.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 522.7 | |
| All* | Total formula weight | 139822.4 |
