9BHW

Septin Tetrameric Complex SEPT7/SEPT9 of Ciona intestinalis by Cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	CiSeptin-7	polymer	419	48769.0	2	UniProt (H2Y169) Pfam (PF00735)	Ciona intestinalis (vase tunicate)
2	B, C (B, C)	Neuronal-specific septin-3	polymer	364	41559.4	2	UniProt (F7AQE7) Pfam (PF00735)	Ciona intestinalis (vase tunicate)	CiSeptin-9
3	E, F, G, H (A, B, C, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)

Sequence modifications

A, D: 10 - 409 (UniProt: H2Y169)

PDB	External Database	Details
Met 1	-	expression tag
Val 2	-	expression tag
Leu 3	-	expression tag
Leu 4	-	expression tag
Asn 5	-	expression tag
Gln 6	-	expression tag
Asp 7	-	expression tag
Met 8	-	expression tag
Ala 9	-	expression tag
-	Met 343	deletion
-	Glu 344	deletion
Asn 410	-	expression tag
Arg 411	-	expression tag
Thr 412	-	expression tag
Leu 413	-	expression tag
Gly 414	-	expression tag
Arg 415	-	expression tag
Lys 416	-	expression tag
Lys 417	-	expression tag
Lys 418	-	expression tag
Trp 419	-	expression tag

B, C: 1 - 356 (UniProt: F7AQE7)

PDB	External Database	Details
Trp 357	-	expression tag
Ser 358	-	expression tag
His 359	-	expression tag
Pro 360	-	expression tag
Gln 361	-	expression tag
Phe 362	-	expression tag
Glu 363	-	expression tag
Lys 364	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	180656.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1772.8
All*	Total formula weight	182429.6

*Water molecules are not included.