9BHT

Septin Hexameric Complex SEPT2/SEPT6/SEPT7 of Ciona intestinalis by Cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F	CiSeptin-2	polymer	445	50766.5	2		Ciona intestinalis (vase tunicate)
2	B, E	CiSeptin-6	polymer	429	49661.6	2	UniProt (F6V5P8) Pfam (PF00735) In PDB	Ciona intestinalis (vase tunicate)
3	C, D	CiSeptin-7	polymer	419	48769.0	2	UniProt (H2Y169) Pfam (PF00735) In PDB	Ciona intestinalis (vase tunicate)
4	A, C, D, F	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
5	B, E	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)

Sequence modifications

B, E: 8 - 413 (UniProt: F6V5P8)

PDB	External Database	Details
Met 1	-	expression tag
Val 2	-	expression tag
Lys 3	-	expression tag
Met 4	-	expression tag
Glu 5	-	expression tag
Lys 6	-	expression tag
Asp 7	-	expression tag
Gly 414	-	expression tag
Lys 415	-	expression tag
Glu 416	-	expression tag
His 417	-	expression tag
Leu 418	-	expression tag
Ala 419	-	expression tag
Met 420	-	expression tag
Ser 421	-	expression tag
Gln 422	-	expression tag
Asn 423	-	expression tag
Lys 424	-	expression tag
Thr 425	-	expression tag
Gly 426	-	expression tag
Lys 427	-	expression tag
Lys 428	-	expression tag
Lys 429	-	expression tag

C, D: 10 - 409 (UniProt: H2Y169)

PDB	External Database	Details
Met 1	-	expression tag
Val 2	-	expression tag
Leu 3	-	expression tag
Leu 4	-	expression tag
Asn 5	-	expression tag
Gln 6	-	expression tag
Asp 7	-	expression tag
Met 8	-	expression tag
Ala 9	-	expression tag
-	Met 343	deletion
-	Glu 344	deletion
Asn 410	-	expression tag
Arg 411	-	expression tag
Thr 412	-	expression tag
Leu 413	-	expression tag
Gly 414	-	expression tag
Arg 415	-	expression tag
Lys 416	-	expression tag
Lys 417	-	expression tag
Lys 418	-	expression tag
Trp 419	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	298394.4
Non-Polymers*	Number of molecules	6
	Total formula weight	2819.2
All*	Total formula weight	301213.6

*Water molecules are not included.