9BHL

Human proton sensing receptor GPR65 in complex with miniGs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	261	30137.0	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	370	40857.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (N)	Nanobody 35	polymer	142	15398.1	1		Lama glama	Nb35
4	D (R)	Psychosine receptor	polymer	348	40627.2	1	UniProt (Q8IYL9) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 65,T-cell death-associated gene 8 protein, GPR65

Sequence modifications

A: 5 - 64 (UniProt: P63092)

PDB	External Database	Details
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Leu -4	-	expression tag
Glu -3	-	expression tag
Val -2	-	expression tag
Leu -1	-	expression tag
Phe 0	-	expression tag
Gln 1	-	expression tag
Gly 2	-	expression tag
Pro 3	-	expression tag
Ser 4	-	expression tag
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 372	Ile 372	engineered mutation
Ile 375	Val 375	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -29	-	initiating methionine
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Leu -22	-	expression tag
Glu -21	-	expression tag
Val -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Gln -17	-	expression tag
Gly -16	-	expression tag
Pro -15	-	expression tag
Glu -14	-	expression tag
Asp -13	-	expression tag
Gln -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Leu -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Gly -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: 1 - 337 (UniProt: Q8IYL9)

PDB	External Database	Details
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Ile -1	-	expression tag
Asp 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127019.9
All*	Total formula weight	127019.9

*Water molecules are not included.