9BGJ

SgrAI mutant K242A endonuclease DNA-bound dimer with Mg2+ and intact primary site DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	SgraIR restriction enzyme	polymer	352	39741.8	2	UniProt (Q9F6L0) Pfam (PF07832)	Streptomyces griseus
2	B, D (C, D)	40-1 DNA	polymer	80	24674.7	2		Streptomyces griseus
3	E, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (A, B)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B: 1 - 339 (UniProt: Q9F6L0)

PDB	External Database	Details
Asp 63	Asn 63	conflict
Ala 242	Lys 242	engineered mutation
Lys 336	Leu 336	conflict
Glu 340	-	expression tag
Asn 341	-	expression tag
Leu 342	-	expression tag
Tyr 343	-	expression tag
Phe 344	-	expression tag
Gln 345	-	expression tag
Ser 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128833.1
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	128881.7

*Water molecules are not included.