9BGI

Activated wild-type SgrAI endonuclease DNA-bound dimer with Mg2+ and cleaved primary site DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	SgraIR restriction enzyme	polymer	352	39783.9	2	UniProt (Q9F6L0) Pfam (PF07832)	Streptomyces griseus
2	B, E (C, E)	40-1 DNA	polymer	18	5547.6	2		Streptomyces griseus
3	C, F (D, F)	40-1 DNA	polymer	22	6722.3	2		Streptomyces griseus
4	G, H, I, J (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	K, L, M, N, O... (A, C, D, B, E...)	water	water		18.0	177	Chemie (HOH)

Sequence modifications

A, B: 1 - 339 (UniProt: Q9F6L0)

PDB	External Database	Details
Asp 63	Asn 63	conflict
Glu 340	-	expression tag
Asn 341	-	expression tag
Leu 342	-	expression tag
Tyr 343	-	expression tag
Phe 344	-	expression tag
Gln 345	-	expression tag
Ser 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	104107.7
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	104204.9

*Water molecules are not included.