9BFU

Cryo-EM structure of Sevenless extracellular domain (composite map of the dimer, pH 4.6)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protein sevenless	polymer	2002	225292.9	2	UniProt (P13368) Pfam (PF00041)	Drosophila melanogaster (fruit fly)
2	C, E (C, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G21290RB)
3	D, F (D, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	G, H, I, J, K... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	20	Chemie (NAG)

Sequence modifications

A, B: 123 - 2110 (UniProt: P13368)

PDB	External Database	Details
-	Arg 2038	deletion
-	Arg 2039	deletion
-	Arg 2040	deletion
-	Arg 2041	deletion
-	Arg 2042	deletion
-	Arg 2043	deletion
-	Arg 2044	deletion
-	Arg 2045	deletion
-	Arg 2046	deletion
His 2111	-	expression tag
His 2112	-	expression tag
His 2113	-	expression tag
His 2114	-	expression tag
His 2115	-	expression tag
His 2116	-	expression tag
Trp 2117	-	expression tag
Ser 2118	-	expression tag
His 2119	-	expression tag
Pro 2120	-	expression tag
Gln 2121	-	expression tag
Phe 2122	-	expression tag
Glu 2123	-	expression tag
Lys 2124	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	450585.8
Branched	Number of molecules	4
	Total formula weight	1989.9
Non-Polymers*	Number of molecules	20
	Total formula weight	4424.2
All*	Total formula weight	456999.9

*Water molecules are not included.