9BE5
Cryo-EM structure of Human Nucleosome collected by EPU on Glacios at 3.3 Angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Histone H3.2 | polymer | 97 | 11431.4 | 1 | UniProt (Q71DI3) Pfam (PF00125) | Homo sapiens (human) | H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o |
| 2 | B, F (B, F) | Histone H4 | polymer | 83 | 9409.1 | 2 | UniProt (A0A9J8D176) Pfam (PF15511) | Homo sapiens (human) | |
| 3 | C (C) | Histone H2A type 1 | polymer | 103 | 11294.1 | 1 | UniProt (P0C0S8) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | H2A.1,Histone H2A/ptl |
| 4 | D, H (D, H) | Histone H2B type 1-J | polymer | 95 | 10607.2 | 2 | UniProt (P06899) Pfam (PF00125) | Homo sapiens (human) | Histone H2B.1,Histone H2B.r,H2B/r |
| 5 | E (E) | Histone H3.2 | polymer | 98 | 11502.4 | 1 | UniProt (Q71DI3) Pfam (PF00125) | Homo sapiens (human) | H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o |
| 6 | G (G) | Histone H2A type 1-B/E | polymer | 105 | 11494.4 | 1 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
| 7 | I (I) | DNA (145-MER) | polymer | 145 | 44520.4 | 1 | Homo sapiens | ||
| 8 | J (J) | DNA (145-MER) | polymer | 145 | 44991.7 | 1 | Homo sapiens |
Sequence modifications
A: 38 - 134 (UniProt: Q71DI3)
C: 16 - 118 (UniProt: P0C0S8)
D, H: 44 - 122 (UniProt: P06899)
E: 38 - 135 (UniProt: Q71DI3)
G: 14 - 118 (UniProt: P04908)
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Val 87 | Ile 88 | conflict |
| Arg 99 | Lys 100 | conflict |
| Ser 113 | Ala 114 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Lys 28 | - | expression tag |
| Thr 29 | - | expression tag |
| Arg 30 | - | expression tag |
| Lys 31 | - | expression tag |
| Glu 32 | - | expression tag |
| Ser 33 | - | expression tag |
| Tyr 34 | - | expression tag |
| Ala 35 | - | expression tag |
| Ile 36 | - | expression tag |
| Tyr 37 | - | expression tag |
| Val 38 | - | expression tag |
| Tyr 39 | - | expression tag |
| Lys 40 | - | expression tag |
| Val 41 | - | expression tag |
| Leu 42 | - | expression tag |
| Lys 43 | - | expression tag |
| Ser 57 | Gly 61 | conflict |
| Val 66 | Ile 70 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 40 | Ser 41 | conflict |
| Val 87 | Ile 88 | conflict |
| Ser 113 | Ala 114 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 175266.9 | |
| All* | Total formula weight | 175266.9 |
