9BDC

Cryo-EM Structure of the TEFM bound Human Mitochondrial Transcription Elongation Complex in a Closed Fingers Domain Conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Transcription elongation factor, mitochondrial	polymer	232	27262.4	2	UniProt (Q96QE5)	Homo sapiens (human)
2	C (E)	DNA-directed RNA polymerase, mitochondrial	polymer	1119	127150.1	1	UniProt (O00411) Pfam (PF14700) Pfam (PF00940)	Homo sapiens (human)
3	D (N)	Non-Template Strand DNA (NT27mt_+1T)	polymer	34	10474.7	1		synthetic construct
4	E (R)	RNA (RNA14mt)	polymer	14	4493.7	1		synthetic construct
5	F (T)	Template Strand DNA (TS31mt_+1A)	polymer	34	10428.7	1		synthetic construct

Sequence modifications

A, B: 236 - 450 (UniProt: Q96QE5)

PDB	External Database	Details
Met 229	-	initiating methionine
Asp 230	-	expression tag
Glu 231	-	expression tag
Asn 232	-	expression tag
Ala 233	-	expression tag
Lys 234	-	expression tag
Glu 235	-	expression tag
Leu 451	-	expression tag
Glu 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag

E: 120 - 1230 (UniProt: O00411)

PDB	External Database	Details
Met 112	-	expression tag
Gly 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
Ala 555	Glu 555	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	207072.0
All*	Total formula weight	207072.0

*Water molecules are not included.