9BCU

Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 52 nt RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase subunit A'	polymer	906	103038.6	1	UniProt (Q5JE33) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo1N
2	B	DNA-directed RNA polymerase subunit B	polymer	1123	127468.0	1	UniProt (Q5JE32) In PDB	Thermococcus kodakarensis
3	C	DNA-directed RNA polymerase subunit A"	polymer	391	43727.4	1	UniProt (Q5JE34) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo1C
4	D	DNA-directed RNA polymerase subunit D	polymer	261	29658.0	1		Thermococcus kodakarensis
5	E	DNA-directed RNA polymerase subunit E	polymer	190	21893.4	1	UniProt (Q5JIY4) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo7
6	F	DNA-directed RNA polymerase subunit F	polymer	122	14519.7	1		Thermococcus kodakarensis
7	H	DNA-directed RNA polymerase subunit H	polymer	82	9522.0	1	UniProt (Q5JE31) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo5
8	K	DNA-directed RNA polymerase subunit K	polymer	60	6583.9	1		Thermococcus kodakarensis
9	L	DNA-directed RNA polymerase subunit L	polymer	94	11013.5	1	UniProt (Q5JE88) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo11
10	N	DNA-directed RNA polymerase subunit N	polymer	65	7602.0	1	UniProt (Q5JJC9) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo10
11	P	DNA-directed RNA polymerase subunit P	polymer	49	5553.7	1	UniProt (Q5JDM8) In PDB	Thermococcus kodakarensis	DNA-directed RNA polymerase subunit Rpo12
12	G	Transcription elongation factor Spt5	polymer	152	16776.4	1	UniProt (Q5JH33) In PDB	Thermococcus kodakarensis
13	I	Transcription elongation factor Spt4	polymer	73	8561.9	1	UniProt (Q5JIY5) In PDB	Thermococcus kodakarensis
14	J, M	Transcription termination factor FttA	polymer	648	73473.7	2	UniProt (Q5JH24) In PDB	Thermococcus kodakarensis	Cleavage and polyadenylation specficity factor subunit homolog
15	5	non-template strand DNA	polymer	36	11096.2	1		synthetic construct
16	6	template strand DNA	polymer	36	11060.1	1		synthetic construct
17	7	RNA (29-MER)	polymer	52	16033.0	1		synthetic construct
18	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
19	M, A, B, N, P...	ZINC ION	non-polymer		65.4	8	Chemie (ZN)

Sequence modifications

I: 1 - 67 (UniProt: Q5JIY5)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

J, M: 1 - 648 (UniProt: Q5JH24)

PDB	External Database	Details
Ala 255	His 255	conflict
Ala 591	His 591	conflict

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	591055.2
Non-Polymers*	Number of molecules	9
	Total formula weight	547.6
All*	Total formula weight	591602.7

*Water molecules are not included.