9BCT
Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 44 nt RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit A' | polymer | 906 | 103038.6 | 1 | UniProt (Q5JE33) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo1N |
| 2 | B (B) | DNA-directed RNA polymerase subunit B | polymer | 1123 | 127468.0 | 1 | UniProt (Q5JE32) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) | Thermococcus kodakarensis | |
| 3 | C (C) | DNA-directed RNA polymerase subunit A" | polymer | 391 | 43727.4 | 1 | UniProt (Q5JE34) Pfam (PF04998) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo1C |
| 4 | D (D) | DNA-directed RNA polymerase subunit D | polymer | 261 | 29658.0 | 1 | Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (Q5JJF4) | Thermococcus kodakarensis | |
| 5 | E (E) | DNA-directed RNA polymerase subunit E | polymer | 190 | 21893.4 | 1 | UniProt (Q5JIY4) Pfam (PF03876) Pfam (PF00575) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo7 |
| 6 | F (F) | DNA-directed RNA polymerase subunit F | polymer | 122 | 14519.7 | 1 | Pfam (PF03874) UniProt (by SIFTS) (Q5JI52) | Thermococcus kodakarensis | |
| 7 | G (H) | DNA-directed RNA polymerase subunit H | polymer | 82 | 9522.0 | 1 | UniProt (Q5JE31) Pfam (PF01191) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo5 |
| 8 | H (K) | DNA-directed RNA polymerase subunit K | polymer | 60 | 6583.9 | 1 | Pfam (PF01192) UniProt (by SIFTS) (Q5JJD0) | Thermococcus kodakarensis | |
| 9 | I (L) | DNA-directed RNA polymerase subunit L | polymer | 94 | 11013.5 | 1 | UniProt (Q5JE88) Pfam (PF13656) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo11 |
| 10 | J (N) | DNA-directed RNA polymerase subunit N | polymer | 65 | 7602.0 | 1 | UniProt (Q5JJC9) Pfam (PF01194) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo10 |
| 11 | K (P) | DNA-directed RNA polymerase subunit P | polymer | 49 | 5553.7 | 1 | UniProt (Q5JDM8) Pfam (PF03604) | Thermococcus kodakarensis | DNA-directed RNA polymerase subunit Rpo12 |
| 12 | L (G) | Transcription elongation factor Spt5 | polymer | 152 | 16776.4 | 1 | UniProt (Q5JH33) Pfam (PF03439) Pfam (PF00467) | Thermococcus kodakarensis | |
| 13 | M (I) | Transcription elongation factor Spt4 | polymer | 73 | 8561.9 | 1 | UniProt (Q5JIY5) Pfam (PF06093) | Thermococcus kodakarensis | |
| 14 | N, O (J, M) | Transcription termination factor FttA | polymer | 648 | 73473.7 | 2 | UniProt (Q5JH24) Pfam (PF17214) Pfam (PF16661) Pfam (PF10996) Pfam (PF07521) | Thermococcus kodakarensis | Cleavage and polyadenylation specficity factor subunit homolog |
| 15 | P (5) | non-template strand DNA | polymer | 36 | 11096.2 | 1 | synthetic construct | ||
| 16 | Q (6) | template strand DNA | polymer | 36 | 11060.1 | 1 | synthetic construct | ||
| 17 | R (7) | RNA | polymer | 44 | 13649.8 | 1 | synthetic construct | ||
| 18 | S (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 19 | AA, T, U, V, W... (M, A, B, N, P...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) |
Sequence modifications
I: 1 - 67 (UniProt: Q5JIY5)
J, M: 1 - 648 (UniProt: Q5JH24)
| PDB | External Database | Details |
|---|---|---|
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 255 | His 255 | conflict |
| Ala 591 | His 591 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 588672.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 547.6 | |
| All* | Total formula weight | 589219.6 |
